Larvae fed the diet with 0.01% FA significantly increased activities of lysozyme and total nitric oxide synthase. Compared with the control, supplementation of FA remarkably increased activities of alkaline phosphatase and leucine aminopeptidase in the brush border membrane of intestine. Diets supplemented with 0.02%–0.04% FA significantly increased the activity of trypsin in the intestinal segment, while 0.01%–0.02% FA significantly increased the activity of trypsin in the pancreatic segment. Moreover, supplementation of FA significantly increased mRNA expression of intestinal epithelial proliferation and barrier genes ( pcna, zo-1 and zo-2). The supplementation of 0.01%–0.02% FA significantly increased the muscular thickness of intestine. Larvae fed the diet with 0.01% FA had significantly higher villus height than the control. Based on the specific growth rate, the optimal supplementation was 0.0135% FA. Meanwhile, supplemental FA significantly increased final body weight and specific growth rate. Results showed that the supplementation of 0.04% FA significantly improved survival rate of large yellow croaker larvae. Four isonitrogenous and isolipids diets containing 0.00%, 0.01%, 0.02% and 0.04% FA were formulated, respectively.
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